高果糖饲料诱导的胰岛素抵抗伴高血压大鼠模型及其特点

Insulin Resistance and Hypertension Rat Model Induced by Fructose-rich and Its Features

目的 建立胰岛素抵抗伴高血压动物模型并观察其特点。方法 用 6周龄的雄性SD大鼠。将大鼠分为 2组 ,正常组对照组 (n =35 )以碳水化合物 6 0 %、脂肪 11%、蛋白质 2 9%的普通标准饲料喂养 ;高果糖餐组 (n=35 )则改以果糖 6 6 %、脂肪 12 %、蛋白质 2 2 %的高果糖饲料喂养。用尾部测量法测定收缩期血压 (SBP) ;用正常血糖高胰岛素钳夹 (euglycemichyperinsulinemicglucoseclamp ,GC)技术评价胰岛素敏感性。结果 4周后两组体重、血压、胰岛素敏感性指数差异均有显著性 ;8周后 ,除以上差异外 ,高果糖餐组TG较正常对照组有显著升高 ,FFA、FINS也有TG一样的趋势 ,但差异无显著性 ,而糖耐量试验、每天平均饲料摄入量两组间无显著差异。结论 高果糖餐喂养的SD大鼠 ,具有典型的高血压伴胰岛素抵抗的特点 ,是研究胰岛素抵抗和高血压理想的动物模型。

Objective The aim of the study was to create a rat model(Fructose Fed Rats, FFR)of insulin resistance (IR) and hypertension ,and to investigate its features. Methods Six week old male Sprague Dawley rats were fed with either normal rat chow groups(60% vegetable starch, 11% fat and 29% protein, n =35) or fructose rich diet groups(66% fructose, 12% fat and 22% protein, n =35).In all rats, The euglycemic hyperinsulinemic glucose clamp(GC)technique was performed to estimate insulin sensitivity. Blood pressure was elevated with tail cuff method. Results It was showed that blood pressure of the FFR groups was significantly increased compared with control groups. The average rate of glucose clamp,as a measure of insulin sensitivity (M value), and weight were significantly lower in FFR compared with control ( P =0.012-0.002) after 4 weeks or 8 weeks. TG was significantly decreased in the control groups compared with FFR groups ( P =0.036).However, FFA and FINS were the same as TG,with no significant diffrence( P =0.066,0.074). Conclusion This rat model is characterized by typical insulin resistance and hypertension. It may provide a advantageous tool for investigations of insulin resistance and hypertension.