摘要
Caffeoyl-Coenzyme A 3-O-Methyltransferase (CCoAOMT) is a key enzyme in lignin biosynthesis pathway. Three rice CCoAOMT genes were identified and designated as OsCOA1, OsCOA20 and OsCOA26. OsCOA1 contains four exons and three introns, while the other two have three exons and two introns. The deduced amino acid sequences of these rice genes share a high identity (75.43%) with other plant CCoAOMT proteins and contain the CCoAOMT specific motifs. Phylogenetic analysis indicates that OsCOA1 has the closest evolutionary relationship to maize CCoAOMT. In contrast, OsCOA20 and OsCOA26 belong to another clade. Northern blot analyses and in situ hybridization studies in- dicate that the three rice CCoAOMT genes are highly ex- pressed in developing sclerenchyma cells and vessel bundles of young leaves, suggesting that they are probably involved in constitutive lignification.
Caffeoyl-Coenzyme A 3-O-Methyltransferase (CCoAOMT) is a key enzyme in lignin biosynthesis pathway. Three rice CCoAOMT genes were identified and designated as OsCOA1, OsCOA20 and OsCOA26. OsCOA1 contains four exons and three introns, while the other two have three exons and two introns. The deduced amino acid sequences of these rice genes share a high identity (75.43%) with other plant CCoAOMT proteins and contain the CCoAOMT specific motifs. Phylogenetic analysis indicates that OsCOA1 has the closest evolutionary relationship to maize CCoAOMT. In contrast, OsCOA20 and OsCOA26 belong to another clade. Northern blot analyses and in situ hybridization studies in- dicate that the three rice CCoAOMT genes are highly ex- pressed in developing sclerenchyma cells and vessel bundles of young leaves, suggesting that they are probably involved in constitutive lignification.
