如何减少浑浊尿对尿液分析仪测定蛋白的影响

How to reduce the infections from turbid urine for determining protein with urinalysis instrument

目的:探讨如何降低浑浊尿对尿液分析仪在测定蛋白的影响。方法:由一位技术熟练的技师将各种不同蛋白的浑浊尿各30例,置室温用水平式离心机以200r/min离心10min后,取其上清液按标本顺序编排好,用尿液分析仪测定。结果:浑浊尿经离心后,前后结果有明显不同,原蛋白()者阴性结果占73.3%,原蛋白()者阴性结果占70%,原蛋白(+)者阴性结果占60%。而没有浑浊的尿,其蛋白基本没什么影响。结论:尿液分析仪测定浑浊尿蛋白结果有明显偏差,采用离心沉淀可大大地排除其影响,具有可行性,能够减少假阳性结果,对临床分析有实际意义。

Objective To discuss how to lower the influence of turbid urine on urinalysis instrument. Method Two skilled technicians centrifugated 30 turbid urine samples of varied protein levels respectively and then placed them in plane centrifugal machine to centrifugate urine with 2000r/min for 10 minutes at ordinary temperature ,taking out their supernatant by sample orders and then analysed them. Results there is a marked difference between the results of turbid urine centrifugation and after that. While former results with +++ level, the negative accounts for 73.3%.as to former results with ++ level ,the negative accounts for 70%, as to results with + level ,the negative accounts for 60% .while clear urine ,the results kept accord with the former ones. Conclusion There was a marked difference on the results of protein in turbid urine when using urinalysis instrument. It eliminated to an extent when centrifugated urine . centrifugation can lower false positive and has its feasibility .it has significance on clinical analysis.