摘要
目的 研究碱性成纤维细胞生长因子 (bFGF)及其受体FGFR1在氪激光诱导的棕色挪威 (BN)大鼠脉络膜新生血管 (CNV)形成中的作用机制。方法 应用氪激光 ( 64 7nm ,3 60mW ,5 0 μm ,0 .0 5s)对 3 0只雄性BN大鼠实验眼进行视网膜光凝 ,分别于光凝后 3、7、14、2 1、2 8和 5 6d摘除眼球进行原位杂交检测bFGFmRNA ,免疫组织化学检测bFGF和FGFR1。结果 光凝 3d后 ,光凝区视网膜内bFGFmRNA和FGFR1蛋白表达水平逐渐下降 (P <0 .0 1)。 7d后视网膜下出现bFGFmRNA和FGFR1染色阳性的CNV ,此后其阳性染色面积及密度逐渐增加 (P <0 .0 1)。结论 bFGF和FGFR1通过自分泌和旁分泌途径在新生血管形成部位结合 ,引起FGFR1自磷酸化 。
ObjectiveTo study the roles of basic fibroblast growth factor(bFGF) and fibroblast growth factor receptor 1(FGFR1) in pathogenesis of choroidal neovascularization(CNV) of Brown Norway(BN) rat induced by krypton laser. MethodsThe experimental eyes of thirty male BN rats received krypton laser(647nm,360mW, 50μm,0.05s)to induce CNV. The retina was processed for expression of bFG FmRNA with in situ hybridization and immunohistochemistry of bFGF and FGFR1 on 3, 7, 14, 21, 28 and 56 days after laser photocoagulation. ResultsThe levels of bFGF mRNA and FGFR1 in retina were decreased after 3 days( P <0 01). CNV was firstly seen on day 7 after laser.The area and density of positively stained cells for bFGF mRNA and FGFR1 were increased during the development of CNV( P< 0.01). ConclusionsbFGF ligand with FGFR1 results in FGFR1 phosphorylation by the way of autocrine and paracrine and further mediates the differentiation of vascular endothelial cells and the formation of CNV.
出处
《眼科研究》
CSCD
北大核心
2003年第3期234-237,共4页
Chinese Ophthalmic Research
基金
全军"十五"科研基金资助 (0 2M0 1 4 )
