铜绿假单胞菌产吩嗪类色素的分离纯化及其对赤潮生物生长的影响

Isolation and Purification of Phenazine Pigments Produced by Pseudomonas aeruginosa and its Effects on the Growth of Red Tide Organisms

.从胜利油田被原油污染的土壤中富集分离得到菌株O 2 2,经鉴定属于铜绿假单胞菌.菌株O 2 2在海水培养基中能生长良好且能分泌色素类代谢产物.细菌发酵液经氯仿萃取并通过硅胶色谱柱分离得到桔黄色和蓝色两种色素.抑藻实验结果表明:黄色色素在较低浓度下对赤潮异湾藻以及具齿原甲藻0201 01两种典型的赤潮生物的生长表现出了明显的抑制作用(EC50<2mg·L-1),而在相同实验条件下,对非赤潮生物青岛大扁藻的生长影响不大.蓝色色素在低浓度下对藻细胞生长影响不明显(EC50>50mg·L-1),但在碱性条件下可经水解转变为黄色色素.可见,该细菌的色素代谢产物在赤潮治理中具有很好的应用前景.进一步借助紫外以及GC MS测试手段对抑藻物质进行了结构分析,结果表明两种色素为吩嗪类化合物,并认为黄色色素的抑藻作用是由于其中含有1 羟基吩嗪.

The bacteria strain O-2-2 was isolated from the contaminated water of Shengli Oilfield, Dongying, Shandong, China, which had subsequently been identified as a species of Pseudomonas aeruginosa. In the seawater medium it could grow well and produce pigments. The algicidal activities of the pigments were investigated. The results indicated that the yellow pigment had potential for the selective control of harmful algal bloom species, H. akashiwo and P. dentatum(EC_(50) <2.0 mg·L^(-1)). Although the blue pigment exhibited no apparent growth inhibitory effect on H.akashiwo(EC_(50)>50 mg·L^(-1)), the yellow pigment could generate from the blue pigment by alkaline hydrolysis. As such, the pigments which were identified as phenazine by UV and GC-MS tests could be considered as a potential algicide.