高效液相色谱法测定酮康唑洗剂中酮康唑的含量

Determination of Ketoconazole in Ketoconazole Lotions by HPLC

目的 建立高效液相色谱法测定酮康唑洗剂中酮康唑的含量方法。方法 以 0 .1mol·L-1磷酸二氢铵溶液 -甲醇 (2 0∶80 )为流动相 ,以氧氟沙星溶液为内标物 ,于 2 35nm波长处测定洗剂中的酮康唑含量。结果 酮康唑线性范围为 10～ 30 0 μg·ml-1,线性关系良好 (r =0 .9997) ,酮康唑的平均回收率为 99.7% ,RSD为 1.4 2 % (n =3)。结论 本方法灵敏 ,准确 ,快捷。

Aim To set up an HPLC method for the determination of Ketoconazole in Lotions.Method Ofoxacin was used as internal standard.The wavelength of detection was 235nm.0.1mol·L -1 ammonium dihydrogen phosphate-methanol(20∶80)was used as mobile phase.Results It was linear in the range of 10～300μg·ml -1 with good corrolation (r=0.999 7).Average recovery was 99.7% (RSD=1.42% n=3).Conclusion The method is sensitive,simple and accurate.