酵母催化2-辛酮不对称还原为2-辛醇

ASYMMETRIC REDUCTION OF 2-OCTANONE TO S-2-OCTANOL BY YEAST IN AQUEOUS PHASE

利用活性酵母细胞催化 2 辛酮的不对称还原 ,合成在制备光学活性物质中具有重要用途的直链手性仲醇 .实验发现不对称还原的产物主要是S 2 辛醇 ,反应的转化率和光学选择性都比较高 .考察了底物浓度、产物、辅助底物的种类及浓度 ,以及 pH值和温度对反应的转化率和光学选择性的影响 .结果表明 ,底物和产物对酵母的活性具有一定的毒害作用 ,产物 2 辛醇对酮的还原反应具有抑制作用 ,酵母能选择性地催化S 2 辛醇氧化为相应的酮 ;在酵母催化还原 2 辛酮的同时需要一定的辅助底物来再生辅酶NAD (P)H ,利用 10g·L-1的葡萄糖作辅助底物较好 ;温度 30℃、pH值 3 0或 7

The asymmetric reduction of 2-octanone to 2-octanol by yeast in aqueous phase was investigated.The reduction product was mainly S-2- octanol.The results indicated that the conversion ratio of 2-octanoe and the enantiomeric excess values of product were both satisfactory for asymmetric reduction.It was shown also that in the catalysis of the ketone reduction the co-substrate was required to regenerate the NAD(P)H.The appropriate condition to the reaction was 30 ℃ and pH 3.0 or pH 7.0. The high concentrations of substrate and product would have some toxicity to the yeast cells and the high concentration of 2-octanol would inhibit the reduction reaction.When too much S-2-octanol existed in the medium, the S-2-octanol would be oxidized to 2-octanone by yeast, which showed that yeast could be a biocatalyst not only for reduction of 2-octanone to S-2-octanol, but also for oxidation of S-2-octanol to 2-octanone.