猪繁殖与呼吸综合症病毒四川株分离、鉴定及其病原特性研究

Studies on the isolation,identification and pathogenic characteristic of Porcine Reproductive and Respiratory Syndrome Virus of Sichuan Strains

从四川地区发病仔猪分离到 3株病毒 (SC1、SC2和SC3) ,电镜下可见病毒呈球形 ,有囊膜 ,病毒能在Marc 14 5细胞上生长并致细胞出现CPE ,直径约 5 5nm ,核酸为RNA ,不凝集猪、黄牛、犬、兔、豚鼠、鸡、鸭、鹅的红细胞 ;pH值 2 ,3,4 ,8,9,10处理 30min和 5 6℃处理 6 0min病毒即失去对Marc 14 5细胞的感染能力。经病毒中和实验、特异性单克隆免疫荧光抗体和特异性RTPCR鉴定表明分离毒为美洲型猪繁殖与呼吸综合症病毒 (PRRSV) ,分离病毒素回归 30日龄仔猪可出现高热和呼吸道症状。研究首次从病原学角度证实美洲型PRRSV在四川的存在 ,利用分离毒制备的疫苗具有良好的免疫原性。

Three strain of virus (SC1,SC2andSC3)were isolated from Sichuan districtThe viruses were sphericity with envelope,about 50nm in diameter and can growth in the Marc145 cell line and induce CPE,Nucleicacid is RNA,The viruses can't agglutinate red cells from pis,scalper,dog,rabbit,guinea pig ,chicken,duck and gooseWhen the viruses deal with PH2,3,4,8,9,10 for 30 min and 56℃ 60 min ,the viruses can't infected Marc145 cell lineThree strain of virus are identified as Porcine Reproductive and Respiratory Syndrome Virus(PRRSV) belong American by observed in electric microscope,immunofluorescent test,virus neutralization test and RTPCR test30 days piglet toob place respiratory signs and high animal heat after inoculated with the isolatesOilemulsion inactivated vaccine made from SC1 isexcellent in immunogenic and excellent laboratory and field experiment proved prevening PRSS