Fenton试剂预处理对纤维素酶解的作用

The Effect of Pretreatment by Fenton Reagents on the Biodegradation of Cellulose

用Fenton试剂反应产生的高反应活性的羟自由基(HO·)处理纤维素材料短绒棉,研究纤维素结构的变化及其对纤维素酶解的影响。实验结果显示,羟自由基作用于纤维素后,能使其产生大量的还原性末端,同时也在一定程度上破坏了纤维素分子间的氢键结构,但是还原性末端产生的数量及氢键破坏的程度与纤维素酶解效率之间没有直接的相关性。由此可见,Fenton试剂作用于纤维素的机制与褐腐菌小分子的Fenton型反应机制明显不同。

Low molecular weight chelators as well as Fenton reagents are believed to play an important role in the early non-enzymatic stage of brown-rot decay of wood. In this study, cellulose materials such as shot-fiber cotton were pretreated by Fenton reagents, which can produce HO·, a kind of high reactive radical. The changes of structure and composition caused by pretreatment were investigated. The digestibility of pretreated cellulose to cellulase was studied. The result indicated that lots of the reducing ends were produced after pretreatment. Many inter- and intra- hydrogen bonds were also disrupted. But all these didn抰 contribute to the digestibility of the cellulose. This indicate that the Fenton reagents does not work in the same way as the low molecular weight chelators produced by brown rot fungi in the biodegradation of cellulose.