毛细管电泳法测定美多芭片剂中的左旋多巴和羟苄丝肼

Determination of levodopa and benserazide in Madopar tablet with capillary electrophoresis

建立了毛细管电泳紫外检测测定美多芭片剂中左旋多巴和羟苄丝肼的方法,优化了缓冲溶液浓度和pH、分离电压和进样时间等条件。在优化的条件下,15min内实现了左旋多巴和羟苄丝肼两种物质的基线分离。左旋多巴和羟苄丝肼分别在006～10mg/mL和005～090mg/mL浓度范围内和电泳峰高成线性关系,左旋多巴和羟苄丝肼的检出限分别为0021mg/mL和0017mg/mL。方法用于分析实际样品,得到令人满意的结果。

A method of determination of levodopa and benserazide in Madopar tablet by capillary electrophoresis with ultraviolet detection was established.The pH and concentration of the running buffer solution,separation voltage and injection time were optimized.Under the optimized conditions,baseline separation of levodopa and benserazide was realized within 15min.There was a good linear relationship between the concentration and the electropherogram peak height of levodopa and benserazide in the 0 06～ 1.0mg/mL and 0.05～0.90 mg/mL concentration range,respectively.Based on three times noise,the detection limits of levodopa and benserazide were 0.021mg/mL and 0.017 mg/mL,respectively.The method was applied to real sample analysis with good results.