SARS冠状病毒S基因序列及细胞抗原表位预测

Comparison of S genome sequence of SARS-CoV and prediction of B cell epitopes

目的 比较SARS冠状病毒各分离株S基因序列及氨基酸序列之间的差异 ,预测SARS -CoV的S蛋白的抗原表位。方法 利用Lasergene软件包中的EditSeq从 2 1株SARS冠状病毒分离株截取S基因序列并翻译成氨基酸序列 ,然后用ClustalX软件对截取序列进行比较分析 ,确定基准株 ,最后用Protean软件对基准株进行抗原表位预测。结果 在 2 1株分离株的S基因中有 8株核苷酸序列、13株氨基酸序列没有发生点突变 ;预测出 78个可能性抗原表位。结论 SARS -CoV的S蛋白相当保守 ,只发生个别点突变 ,可能性抗原表位多 ,且在其中的 14个区域中可能性最显著。

Objective To compare the differences of S genome and amino acids sequence f SARS-CoV isolates,analyze possible B cell epitopes of S protein.Methods S genome sequences of SARS-CoV isolates are cut with Editseq program of Lasergene and then translated into amino acids sequences.To compare the difference of genome and amino acids sequences with Clustal X and then predict B cell epitopes with Protean program.Results There are not point mutations in 8 genome sequences and 13 amino acids sequences of the S gene of 21 isolates and 78 of B cell epitopes in S protein are predicted.Conclusion S genome sequences of SARS-CoV are rather conservative and only change a little.There are many possible antigenic epitopes in S protein,especially in 14 zones.