SOD基因表达与衰老的相关性

Correlation of SOD gene expression to senility

目的 探讨SOD活性及SOD基因表达与衰老的相关性。方法 选用快速老化模型鼠 (SAM -P/ 8)进行试验 ,采用生物化学及RT -PCR方法 ,从亚细胞及分子水平上测定衰老过程中丙二醛 (MDA) ,超氧化物歧化酶(SOD)及其基因表达的变化情况。结果 SAM -P/ 8肝组织中SOD活性随着年龄的增加而逐渐降低 ;而MDA含量随年龄的增加逐渐增高 ,有显著性差异 (P <0 0 5 )。不同月龄SAM -P/ 8肝细胞中Cu -ZnSOD基因表达随年龄的增加Cu -ZnSODmRNA逐渐降低 (P <0 0 1)。结论 SOD活性与SOD基因表达的增龄变化有明显的相关性 ,SOD基因转录活性的降低可能是导致SOD活性降低的重要原因 ,表明SOD在衰老过程中扮演着重要角色。

Objective To explore the changes on activity and expression of SOD in the progress of senescence.Methods Senescence-accelerated mouse p/8 (SAM-P/8) was used in the study.Biochemical assay and Reverse Transcription Polymerase Chain Reaction (RT-PCR) were adopted to measure changes occurred on SOD activity and gene expression in the progress of senescence.Results Activity of SOD decreased significantly with aging (P<0 05),while the level of MDA increased significantly with aging (P<0 05).And the level of Cu-Zn SOD mRNA derived from the liver of SAM-P/8 decreased with aging (P<0 01).Conclusion Activity of SOD gradually decreased with aging.The decreasing expression of Cu-Zn SOD gene was an important reason for SOD's decreasing activity.It could be inferred that SOD played an important role in the progress of aging.