一氧化氮对约氏疟原虫裂殖子感染力的影响

Research on effect of nitric oxide on infectivity of merozoite of plasmodium yoelii

目的 探讨一氧化氮 (NO)对红内期疟原虫裂殖子感染力的影响。方法 利用NO发生剂 (NOC) 5和NOC18于体外预处理约氏疟原虫裂殖体 ,然后接种小鼠 ,尾静脉采血 ,吉姆萨薄血膜染色 ,计数小鼠感染率。结果 伴随NOC处理浓度的升高或处理时间的延长 ,小鼠出现感染的时间明显滞后 ,感染率显著下降。结论 NO具有明显削弱约氏疟原虫裂殖子感染力的作用 ,这种效应表现为剂量依赖性和时间依赖性。

Objective To clarify effect of nitric oxide on infectivity of merozoite.Methods P.yoelii schizonts collected and treated with NO donors (NOC5 and NOC18) in vitro,were injected into abdominal cavity of mice.Infective rate was assessed by the microscopic examination of Giemsa-stained smears of tail blood.Results As concentration of NO donor was hoisted and treated time was prolonged,the infective rate of mice decreased obviously and infection occurred late.Conclusion Exogenous nitric oxide was able to weaken infectivity of P.yoelii merozoite in a time and dose dependent manner.