婴幼儿血管瘤和血管畸形组织中一氧化氮合酶的表达及意义

The expression and significance of nitric oxide synthases in hemangiomas and vascular malformations in infants

目的:研究婴幼儿血管瘤和血管畸形组织中一氧化氮合酶(NOS)的表达差异及其意义。方法:采用免疫组织化学方法及图像分析,检测49例血管瘤和29例血管畸形组织中NOS1、2、3蛋白的表达。分别采用秩和检验的Mann-Whitney test和Kruskal-Wallis test法,比较两组和多组等级变量间的差异;使用标准正态分布统计量Z、X2确定P值,或直接用精确概率法计算P值,P<0.05为有显著性差异。全部资料的统计分析均使用SPSS8.0统汁软件包完成。结果:49例血管瘤组织中,NOS1、2、3蛋白的阳性率为分别为2％、38.8％和38.8％;29例血管畸形组织中,NOS1、2、3蛋白的阳性率分别为0、3.5％和3.5％;血管瘤组织中NOS2、3蛋白的阳性表达率均明显高于血管畸形(P<0.001)。增生期血管瘤组,NOS2、NOS3蛋白的阳性率及消退期血管瘤组NOS3蛋白的阳性率均明显高于血管畸形组(P值分别为0.000、0.000和0.007)。年龄<13个月组和<13-24个月组,血管瘤的NOS2蛋白的阳性表达率均明显高于>24个月组(p分别为0.009和0.003)。结论:血管瘤与血管畸形中NOS蛋白的表达不同,提示两者在病理生理方面存在差异。

PURPOSE:To investigate the difference and significance of nitric oxide synthases (NOS) expression in hemangioma (HA) and vascular malformation (VM) in infants. METHODS:Immunohistochemical staining was used to detect the expression of NOS1,2,3 proteins in 49 cases of HA and 29 cases of VM. Mann-Whitney test and Kruskal Wallis test were used for comparing the differences.The data were analysed statistically with SPSS8.0 package. RESULTS: Positive rates of NOS 1,2,3 proteins in 49 cases of HA was 1%,38% and 38% respectively, in 29 cases of VM was 0%,3% and 3% respectively. The expressive rates of NOS2,3 proteins of HA was significantly higher than that of VM (P<0.001). The positive rates of NOS2,3 proteins in HA of proliferative phase was significantly higher than that in VM (P=0.000). The positive rate of NOS3 protein in HA of involuting phase was significantly higher than that in VM (P= 0.007). The positive rates of NOS2 protein in HA patients below 13 months and between 13 and 24 months was significantly higher than that in HA patients over 24 months (P=0.009 and 0.003). CONCLUSIONS: Expression of NOS in HA is different from that in VM, which indicates that there is pathophysical difference between HA and VM.