Sebia-HYDRASYS电泳系统在血清蛋白电泳中的应用评价

Application of Serum Protein of Electrophoresis in Sebia-HYDRASYS System

目的 :探讨Sebia HYDRASYS电泳系统在血清蛋白电泳中的应用。方法 :采用法国Sebia$CHY DRASYS电泳系统进行血清蛋白琼脂糖凝胶电泳 ,利用pH(8.8± 0 .1)Tris巴比妥缓冲液作电泳缓冲液 ,血清蛋白质根据其所带电荷数将其分离成ALB、α1、α2 、β、γ球蛋白 ,已分离的蛋白质由氨基黑染色 ,多余染色在酸性介质中被洗去 ,被分离的着色的蛋白质通过光密度仪扫描获得各区较为精确的相对值 ,并与醋酸纤维薄膜电泳法相比较。结果 :琼脂糖凝胶电泳法批内精密度 (CV)为 0 .72 % (白蛋白 )至 4 .98% (α1-球蛋白 )之间 ,总精密度 (CV)为 2 .5 2 % (白蛋白 )至 6 .0 3% (γ球蛋白 )之间 ,较醋酸纤维薄膜电泳法显示较好的精密度 ,琼脂糖凝胶电泳还显示出独特的区带扫描特征。结论 :Sebia-HYDRASYS电泳系统可显示出独特的区带扫描特征 ,分辨率高 ,精密度好 ,简便快速等优点。

Objective To study the serum protein of electrophoresis in Sebia-HYDRASYS system. Methods Detection the serum protein by Agarose gel electrophoresis with Sebia HYDRASYS, using PH(8.8±0.1)Tris barbital buffer for electrophoresis buffer, ALB、α_1、α_2、β、γ globulin were separated by Agarose gel electrophoresis with its electricity ,the separated proteins were dyeing by amidoblack stain , the excrescent dye washed out in acidity medium , the stained proteins were obtained the accurate comparatively result by the optical density scanner and compare with the cellulose acetate membrane electrophoresis. Results The batch precision (CV) were 0.72 % (albumin) to 4.98 % (α_1- globulin),The total precision were 2.52 % (albumin) to 6.03 % (gamma globulin).The precision was better than the cellulose acetate membrane electrophoresis. The method could diplay the unipue zone.Conclusion The Sebia-HYDRASYS system could diplay the unipue zone ,the method is high distinctive , better precision, simple and fast.