表面活性剂协同提取芦丁研究

STUDY ON EXTRACTION OF RUTIN ASSISTED BY SURFACTANT

对不同表面活性剂协同芦丁提取的效果进行了比较 ,筛选出对芦丁提取具有较好协同作用的表面活性剂———吐温 2 0 (Tween 2 0 )。通过试验 ,得到了Tween 2 0协同提取芦丁的最佳工艺条件 :提取两次 ,每次槐米与水、硼砂及Tween 2 0的质量比为 10 0∶70 0∶0 .75∶0 .5 ,在 6 0℃下提取 30min。合并提取液 ,用盐酸调 pH值至 2～ 3,静置 4 0min ,分离出沉淀物 ,干燥 ,即得芦丁 ,产率为 17.5 % ,纯度为 92 .5 %。与传统碱水提取法相比 ,Tween 2 0协同芦丁提取法能缩短提取时间和沉淀时间并提高芦丁产率。

Different surfactants were used as assistants to extract rutin from Flos sophorae,from which Tween 20 was confirmed as the best surfactant. The optimal technology is obtained as follows: extracting twice at (60 ℃) for 30 min,each time with a mass ratio of material to solvent, Tween 20 and borax by 100∶700∶0.75∶0.5,adjusting the extracted liquor′s pH value to 2-3 with 15 % hydrochloric acid,laying aside for 40 min to deposit completely followed by separating out the precipitate.Rutin product can be obtained at yield 17.5 % and purity 92.5 % after drying of the precipitate. Compared with traditional alkaline liquor extraction, the extraction assisted with Tween 20 has the advantages of decreased extracting time and depositing time,and increased yield.