山核桃树皮中生理活性成分的研究(1)

The study on the biologically active components of the bark of Juglans mandshurica Maxim

将山核桃树皮粉碎后用无水乙醇浸泡,乙醇浸出液用正己烷萃取后,制得正己烷提取物。正己烷提取物经柱层析分离后,用高压液相色谱分离得到了一种白色针状晶体化合物,经红外光谱,核磁共振光谱鉴定,推测该化合物为?-谷甾醇,其含量占正己烷提取物重量的0.36%。

The bark of Juglans mandshurica Maxim was extracted by ethanol, After the ethanol extracts was extracted by n-hexane, the yield of the n-hexane extracts is 1.9%. After the n-hexan extuacts was separated with the chromatography , it was further separated by HPLC to gave a white needle compounds. The structure of the compound was determined by IR and 1H-NMR and 13C-NMR, the compound is???sitosterol.The yield of ??sitosterol is 0.36% in n-hexane extracts .