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基因枪介导小麦基因转化体系的建立 被引量：4

Establishment of a Microprojectile Bombardment-Mediated Transformation System for Wheat Cultivar Yumai 18
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摘要 以豫麦18幼胚为受体,建立高效基因枪介导转化体系,经除草剂抗性和对目的基因的PCR、PCR-Southern检测,获得了20株转基因植株,转化率高达3.7%,为基因枪高效介导提供了成功的证据。 An efficient microprojectile bombardment-mediated transformation system was established for wheat Yumai 18, an elite wheat cultivar, newly developed in Henan for its wide adapbility. The transformation rate was as high as 3.7%. A total of 20 transgenic plants were obtained and confirmed by herbicide-resistance assays, PCR and PCR-Southern analysis.
作者 任江萍 李磊 王新国 尹钧
机构地区 河南农业大学国家小麦工程技术研究中心
出处 《扬州大学学报（农业与生命科学版）》 CAS CSCD 2004年第3期38-41,共4页 Journal of Yangzhou University：Agricultural and Life Science Edition
基金 河南省杰出人才创新基金资助项目(12100800)
关键词 小麦 基因枪介导转化 转化率 wheat genegun-mediated transformation transformation efficency
分类号 S512 [农业科学—作物学]
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参考文献8

  • 1Blechl A E,Olin D A.Expression of a novel high-molecular-weight gluteninsubunit gene in transgenic wheat [J].Nature Biotechnology,1996,14:875-879.
  • 2Bliffeld M,Mundy J,Potrykus I,et al.Genetic engineering of wheat for increased resistance to powdery mildew disease [J].Theor Appl Genet,1999,98:1079-1086
  • 3Fennel S,Bohorova N,Van Ginkel M,et al.Plant regeneration from immature embryos of 48 elite CIMMYT bread wheats [J].Theor Appl Genet,1996,92:163-169.
  • 4Mathias R J,Simpson E S.The interaction of genotype and culture medium on the tissue responses of wheat callus [J].Plant Cell Tiss Org Cul,1986,7:31-37.
  • 5Becker D,Horst L.Production of fertile transgenic wheat by microprojectile bombardment [J].Plant Tiss Cell Org Cul,1994,5:299-307.
  • 6Vasil V,Castillo A M,Fromm M E,et al.Herbicide resistance fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus [J].Bio/Technology,1992,10:667-674.
  • 7任江萍,尹钧,师学珍,姜小冬,王智琴.小麦转基因再生植株培养体系的优化[J].华北农学报,2003,18(1):22-25. 被引量：17
  • 8Schurmann P,Jacquot J P.Plant thioredoxin system revisited [J].Annu Rve Plant Physiol Mol Biol,2000,51:371-400.

二级参考文献7

  • 1[1]Vasil V,Castillo A M,Fromm M E,et al.Herbicide resistance fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus[J].Bio Technology,1992,10:667-674.
  • 2[2]Maddock S E,Lancaster V A,Risitoo R,et al.Plant regeneration from cultured immature embryos and inflorescences of 25 cultivars of wheat(Triticum aestivum L.)[J]. J Exp Bot,1983,34:915-926.
  • 3[3]Mathias R J,Simpson E S.The interaction of genotype and culture medium on the tissue responses of wheat callus[J].Plant Cell Tiss Org Cul,1986,7:31-37.
  • 4[4]Fennel S,Bohorova N,Van Ginkel M,et al.Plant regeneration from immature embryos of 48 elite CIMMYT bread wheats[J].Theor Appl Genet,1996,92:163-169.
  • 5[5]Murashige T,Skoog F.A revised medium for rapid growth and bioassays with tobacco tissue cultures[J].Plant Physiol,1962,15:473-497.
  • 6[6]Van Ark H F,Zaal M A C M,Creemers-Molenaar J,et al.Improvement of the tissue culture response of seed-derived callus culture of Poa pratensis L:effect of gelling agent and abscisicacid[J].Plant Cell Tiss Org Cult,1991,27:275-280.
  • 7[7]Etienne H,Sotta B,Montoro P,et al.Relations between exogenous growth regulators and endogenous indole-3-acetic acid and abscisic acid in the expressions of somatic embryogenesis in Hevea brasiliensis[J].Plant Science,1993,88:91-96.

共引文献16

同被引文献46

引证文献4

二级引证文献6

扬州大学学报（农业与生命科学版）
2004年 第3期

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