H-2半相合小鼠EL9611红白血病模型的建立

ESTABLISHMENT OF H-2 HAPLOIDENTICAL MOUSE ERYTHROBLASTIC LEUKEMIA MODELS OF EL9611

①目的 探讨建立EL96 1 1红白血病F1小鼠模型的可行性 ,并为研究移植物抗白血病 (GVL)效应提供模型。②方法 将 1 0 4～ 1 0 7个EL96 1 1细胞分别接种F1小鼠 ,观察生存时间 ,对濒死小鼠取肝、脾和骨髓做病理检查。接种F1小鼠腹腔注射环磷酰胺和阿糖胞苷 (剂量分别是 5 0mg·kg-1 ·d-1 和 6 0mg·kg-1 ·d-1 ) ,连用 6d ,观察化疗敏感性。51 Cr释放实验测定T细胞对EL96 1 1的细胞毒作用。③结果 F1小鼠接种 1 0 4～ 1 0 7个EL96 1 1细胞后均发病 ,EL96 1 1细胞接种量与小鼠生存时间呈负相关 (r =- 0 .91 ,P <0 .0 1 )。肝、脾和骨髓是EL96 1 1细胞常见浸润部位。接种F1小鼠对环磷酰胺和阿糖胞苷灵敏度分别为 2 35 %和 1 97%。T细胞对EL96 1 1细胞的杀伤作用明显高于对照。④结论 接种 1 0 4～ 1 0 7个EL96 1 1细胞可建立EL96 1 1 F1小鼠红白血病模型 ;T细胞对EL96 1

Objective To explore the establishment of EL9611-F1 mouse erythroblastic leukemia models and to supply the models for the study of graft versus leukemia(GVL) effect in H-2 haploidentical transplantation. Methods 10 4-10 7 erythroblastic leukemia cells of EL9611 were respectively inoculated into F1 mice. The survival time was observed. The liver, spleen and bone marrow were obtained from the dying EL9611-F1 mice for pathological examination. Cyclophosphamide(CTX 50 mg·kg -1 ·d -1 ) and arabinosylcytosine (Ara-c 60 mg·kg -1 ·d -1 ) were intraperitoneally injected to the inoculated F1 mice for six days to detect their sensitivity to chemotherapy. Cytotoxic effect of T lymphocyte on EL9611 was detected by 51 Cr release test. Results All F1 mice died of erythroblastic leukemia when inoculated 10 4-10 7 EL9611. There was a negative correlation of inoculated EL9611 quantities and EL9611-F1 mice survival time ( r=-0.91,P <0.01). EL9611 mainly infiltrated the liver, spleen and bone marrow. Sensitivity of EL9611-F1 mice to CTX and Ara-c was 235% and 197%, respectively. Cytotoxic effect of T lymphocytes was significantly higher on EL9611 than on the controls. Conclusion EL9611-F1 mouse erythroblastic leukemia models can be established by inoculating 10 4-10 7 EL9611. T lymphocyte is cytotoxic to EL9611.