微流控芯片用于流式细胞术的基础研究

Studies on the Method of Flow Cytometry Integrated with Microfluidic Chip

自行设计并加工了玻璃微流控芯片 ,并将其与流式细胞术相结合 ,采用羟丙基甲基纤维素 ( HPMC)的磷酸盐溶液为缓冲体系和鞘液 ,解决了微粒在微芯片中流动的若干问题 ,使其状态可以得到更有效的控制 .采用自行组装的激光诱导荧光装置并结合动电聚焦技术 ,实现了对荧光微球的计数 ,并可通过荧光倒置显微镜实时观察到微通道内微球的实际流动情况 .方法简单 ,操作方便 ,并且具有仪器体积小。

A microfluidic chip is designed and fabricated, and coupled with flow cytometry. By using Na 2HPO 4 solution added with hydroxypropyl methyl cellulose(HPMC) as buffer and sheathing fluid, the problems met with the flowing of microspheres in the microchannel were solved, and the fluidic conditions were under more effective control. By using a home-made laser induced fluorescence system and electrokinetic focusing, the counting of the microspheres and real time observation of their actual fluidic state through an inverted fluorescence microscope is made. The whole system is compact and easy to be operated. The comsumption of reagent and sample is little and the speed of the assay is quick.