美托洛尔分子印迹聚合物识别性能的研究

Preparation and Properties of A Metoprolol Molecularly Imprinted Polymer

采用紫外光引发的分子印迹技术,以美托洛尔为模板,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,成功制备了以美托洛尔(Metoprolol)为模板的分子印迹聚合物。结合底物的实验表明,该聚合物对美托洛尔具有优良的吸附选择性。并通过Scatchard方程研究了印迹聚合物对模板分子的结合特性,结果表明,功能单体甲基丙烯酸与模板分子在自组装过程中通过氢键和离子键形成了2类非等价的结合位点,并计算出2类不同结合位点的离解常数分别为Kd1=2 398×10-4mol L,Kd2=6 364×10-4mol L;最大表观结合量Qmax1=58 57μmol g;Qmax2=86 13μmol g;分别为理论吸附量的50 53%和74 31%。

A molecularly imprinted polymer (MIP) was synthesized successfully through a molecular imprinting technique by using Metoprolol as templates, methacrylic acid as a functional monomer and elhylene glycol dimethacrylate as cross-linker,under the condition of UV initiation. The experiments of binding different substrates indicated that the MIP possessed better selectivity toward the template than other analogs. Scatchard analysis showed that the template polymer using methacrylic acid(MAA)as functional monomer could form two kinds of non-equivalent binding sites. The dissociation constants of MIP were estimated to be K-(d1)=2.398×10^(-4) mol/L and K-(d2)=6.364×10^(-4) mol/L.The most apparent adsorption amounts were calculated about Q-(max 1)=58.57 μmol/g and Q-(max 2)=86.13 μmol/g, which correspond to 50.53% and 74.31%of theoretical values,respectively.