首次测得蝴蝶兰基腐病菌rDNA ITS区序列

First Sequencing the ITS of rDNA of Fusarium spp.Isolated from Cymbidium spp.

用Whiteetal.于1990年设计的引物ITS4和ITS5对蝴蝶兰基腐病菌(Fusariumspp )的rDNAITS区片段进行了PCR扩增,并将扩增产物克隆到大肠杆菌JM109中进行测序,测得的序列长度为565bp。该序列的测得为蝴蝶兰基腐病菌的诊断提供了有利证据。

The internal transcribed spacer(ITS) of ribosomal DNA of Fusarium spp. isolated from Cymbidium spp. was amplified by using primers ITS5 and ITS4 (designed by White et al. in 1990). The PCR product was cloned into E. coli JM109 for sequencing. The length of the sequence is 565 bp. This sequence will be helpful for the identification of the pathogen isolated from Cymbidium spp. rot root.