假单胞菌Y8产壳聚糖酶的培养条件

Cultural Conditions of Chitosanase Produced

对假单胞菌Y8产壳聚糖酶培养条件进行研究,得到一个比较优化的培养条件为:pH值6.5,温度32℃,壳聚糖3g/L,酵母膏质量分数0.3%,培养时间3d.不同金属离子及不同诱导物对酶合成的影响表明:Fe2+激活作用显著,而Mg2+,Zn2+,Mn2+则具有一定的抑制作用,壳聚糖和氨基葡萄糖对壳聚糖酶的产生都有较好的诱导作用.

The cultural conditions of chitosanase produced by Pseudomonas Migula Y8 were investigated. The optimal conditions for chitosanase produced by Pseudomonas Migula Y8 were as follows: pH 6.5 , temperature 32 ℃, Chitosan 3 g/L, yeast extract 0.3%, and fermentation for 3days. Fe^(2+)can activate the Chitosanase's production, while Mg^(2+), Zn^(2+)and Mn^(2+) inhibit the production. The better inducer was Chitosan and GlcN.