一氧化氮、前列腺素E_2在先兆早产中的作用

The effects of nitric oxide and prostaglandin E_2 during thereatened preterm labor.

目的 探讨一氧化氮 (NO)、前列腺素E2 (PGE2 )在先兆早产中的作用。方法 采集先兆早产孕妇 ( 2 4例 )外周血和阴道冲洗液 ,采用硝酸还原酶法检测NO水平 ,用放射免疫法测定其PGE2 值 ,并与同孕期对照组 ( 2 4例 )进行比较。结果 先兆早产组血清NO、PGE2 、阴道冲洗液NO和PGE2 水平均高于对照组 (P <0 0 5 ) ,但外周血和阴道冲洗液NO/PGE2 比值均小于对照组 (P <0 0 5 )。以两组孕妇为一个整体研究 ,结果 ,血和阴道冲洗液间NO水平尚不能认为相关 ,而两种样本PGE2 水平呈部分正相关 (r =0 5 2 6,P <0 0 5 ) ,两种样本NO/PGE2 比值间呈部分正相关 ,但相关强度低 (r=0 2 90 ,P <0 0 5 )。结论 先兆早产时孕妇外周血和阴道冲洗液NO、PGE2 增加 ,NO/PGE2 比值下降。NO、PGE2 的上述变化 ,可能是通过诱发宫缩和促使宫颈成熟而成为先兆早产发生的机理之一。

Objective To investigate the effects of nitric oxide (NO) and prostaglandin E 2(PGE 2)during threatened preterm labor.Methods Using nitrate reductase method and radioimmunoassay respectively,the levels of NO and PGE 2 in peripheral blood and vaginal lavage were measured.All the results were compared between threatened preterm labor group (n=24) and preterm control group (n=24).Results Compared with preterm control group,the levels of NO and PGE 2 in threatened preterm labor group were higher (P<0.05),but the ratio of NO and PGE 2 was lower (P<0.05),either in peripheral blood or in vaginal lavage.After analysing the forty-eight pregnant women as a whole,we found that there was not a correlation between serum NO and vaginal lavage NO,but there was positive correlation between plasma PGE 2 and vaginal lavage PGE 2 (r=0.526 P<0.05).Correlation was significant at the 0.05 levels (2-tailed) between blood NO/PGE 2 ratio and vaginal lavage NO/PGE 2 ratio,but correlation coefficient was only 0.290.Conclusion The elevated levels of NO and PGE 2,and decreased NO/PGE 2 ratio were found in peripheral blood and vaginal lavage of patients with threatened preterm labor.These changes of NO and PGE 2 may be involved in the initiation of threatened preterm labor by inducing uterine contractions and facilitating cervical softing and ripening.