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百日咳毒素的等电点测定及层析分离 被引量：2

The pI determination and purification of pertussis toxin
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摘要 百日咳毒素是百日咳杆菌(Bordetellapertussis)产生的一种主要毒力因子,也是百日咳菌苗的主要成分之一.用等电聚焦法测得其等电点为pH6 8.将百日咳杆菌液体培养液在等电点盐析后,通过DEAE-Sephadex-A50阴离子交换剂进一步分离纯化了百日咳毒素.ELISA测定其比活力表明,3批样品的提纯倍数分别为1 47,1 37和1 36. Pertussis toxin is main toxic factor produced by Bordetella pertussis.It is main composition of pertussis vaccine.In this study,the pI examined by isoelectric focusing is pH6.8.At pH6.8 the pertussis medium supernatant is salt out,PT is further purified by chromatography on DEAE-Sephadex-A50 column.The specific activity is determined by ELISA.The purification multiple are 1.47,1.37 and 1.36 separately for three samples.
作者 刘国安 杨晓明 葛瑞昌 何长民
机构地区 西北师范大学生命科学学院 卫生部生物制品研究所 兰州大学生命科学学院
出处 《西北师范大学学报（自然科学版）》 CAS 2004年第4期68-70,共3页 Journal of Northwest Normal University(Natural Science)
关键词 百日咳毒素 等电点 DEAE-Sephadex-A50 pertussis toxin isoelectric point DEAE-Sephadex-A50
分类号 Q503 [生物学—生物化学]
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参考文献11

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二级参考文献7

共引文献22

同被引文献14

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西北师范大学学报（自然科学版）
2004年 第4期

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