中国梨品种S基因型鉴定的初步研究

Study on identification of S-genotypes of Chinese pear cultivars

采用PCR技术和聚丙烯酰胺凝胶电泳法对6个中国梨品种的S基因型进行了鉴定研究,并与已知S基因型的日本梨品种进行了比较。研究结果表明,供试的6个中国梨品种S基因型均不相同,'西子绿'、'金花'和'金水酥'各包含了S1～S7以外的新的S基因,为这些品种田间授粉品种的选配提供了参考。

PCR and PAGE were used to determine the S-genotypes of six Chinese pear cultivars compared with Japanese pear cultivars of known S-genotypes. Results indicated that six Chinese pear cultivars tested varied in S-genotype with one another, three of which, 'Xizilu', 'Jinhua' and 'Jinshuisu' had a new S-gene other than S_1～S_7 -gene respectively, which could facilitate the selection of pollinizers in orchard.