乙肝病毒DNA疫苗的构建及其诱导小鼠的免疫应答

Construction of Hepatitis B DNA Vaccine and Immune Response in Mice Induced by the Vaccine

构建含adr亚型HBV表面抗原基因的核酸疫苗 ,考察人白细胞介素II基因及重组白细胞介素II的免疫佐剂作用。用含有人白细胞介素II基因的真核表达质粒及基因重组白细胞介素II蛋白作为佐剂 ,将编码乙型肝炎病毒表面抗原的重组真核表达质粒 pVAX/HBS免疫BALB/C小鼠 (试验组 ) ,同时设置注射质粒pVAX的阴性对照组 ,并分别于第 2 ,4周后加强免疫各 1次。试验组在第 4周时开始有HBsAb产生 ,阴性对照组未测到HbsAb ,试验组和对照组均未检测到HBsAg。乙肝病毒DNA疫苗能引起小鼠特异性体液免疫应答 ,白细胞介素II的真核表达质粒的佐剂作用不明显 ,基因重组白细胞介素II蛋白具有提高小鼠对乙肝病毒核酸疫苗免疫应答水平的佐剂活性。

A DNA vaccine containing hepatitis B virus surface antigens (adr subtype) was constructed and the adjuvant effect in the vaccine of human IL-2 and IL-2 gene were observed. By using the recombinant IL-2 or the recombinant eukaryotic plasmid containing IL-2 gene as adjuvant, the recombinant eukaryotic plasmid containing HBsAg gene (pVAX/HBS) was injected into BALB/C mice (the tested group). The other BALB/C mice were injected with plasmid pVAX as the negative control group. Then the animals were immunized on the second and forth weeks after the first injections, respectively. The HBsAb in mine of the tested group was detectable on the forth weeks after the final immunization, but in mice of the negative control group was not. HbsAg in mice of both the tested and negative control groups were undetectable. In conclusion, the HBV DNA vaccine could cause specific humoral immune response in mice. And the recombinant IL-2 had adjuvant ability to increase the immune response level in mice against the HBV DNA vaccine, but the recombinant eukaryotic plasmid containing IL-2 gene did not.;