黄海繁茂膜海绵中微生物多样性的研究

Bacteria Diversity Associated with the Marine Sponge Hymeniacidon perleve in China Yellow Sea

构建了中国黄海繁茂膜海绵中细菌 16SrDNA克隆 ,对其遗传多样性进行了分析 ,发现海绵中相关细菌 16SrDNA基因主要归类于紫硫细菌门 (Proteobacteria)中的α_亚门、γ_亚门 ,和放线菌门 (Actinobacteria)等类群。所获得的16SrDNA序列与GenBank中的已知序列差异较大 ,反映出该海绵存在尚未发现的微生物新信息。

The bacteria distribution and phylogenetic diversity of the marine sponge Hymeniacidon perleve, collected from China Yellow Sea were studied by TEM and 16S rDNA sequencing respectively. The phylogenetic affiliation of sponge-associated microorganisms was determined by 16S rDNA sequencing of cloned DNA fragments. 21 clones were found to be unique with 7 attributed to eukaryotes,the other 14 of isolated 16S rDNA sequences were attributed to be α-Proteobacteria(6)、γ-Proteobacteria(7) and Actinobacteria(1). The similarity values for 9 of the clones and the reference sequences blasted in the GenBank were below 95%. In the phylogenetic trees, most of the isolated clones were clustered in one group and differentiated clearly from the other reference sequences. The results indicated that all the 14 isolates were candidates for new genus or species of bacteria associated with Hymeniacidon perleve.