转Bt基因棉花杀虫晶体蛋白的表达及光合特性的研究

Photosynthetic characters and Bt toxin content of different transgenic Bt cottons

转Bt基因棉花 (GK、ZK)及非Bt基因棉花 (CZ)杀虫晶体蛋白表达及光合特性的研究表明 ,杀虫晶体蛋白在转Bt基因棉花GK与ZK中的表达总量及在各器官中的分配均有所不同 .转Bt基因棉花叶片的净光合速率的光响应与常规棉有所不同 .转Bt基因棉花GK与ZK叶片的叶绿素含量、净光合速率、蒸腾速率的日变化有明显的不同 ,而胞间二氧化碳浓度、气孔限制值、叶温的日变化趋势则基本一致 .胞间二氧化碳浓度的日平均值在两转Bt基因棉花间的差异达显著水平 ,而其它各指标在不同处理间的差异均未达显著 .

The study on the photosynthetic characters and Bt toxin content of different Bt cottons(ZK,GK)and non-Bt cotton(CZ)indicated that Bt cottons GK and ZK had different total expressed amount of Bt toxin and its distribution in different tissues.The Bt cottons had obviously different chlorophyll contents,responses of photosynthetic rate to photosynthetic active radiation,and diurnal changes of photosynthetic rate and transpiration rate,but the diurnal change of leaf temperature,CO 2 concentration inside leaf cells and stomatal limit values were approximately the same.There was a significant difference between two transgenic Bt cottons in the diurnal average value of CO 2 concentration inside leaf cells,but the differences in other characteristics were not significant among different cottons.