DNA加合物的形成、诊断与污染暴露指示研究进展

Research advances in DNA adducts:Their formation,diagnosis and pollution-exposure indication

随着人们对污染生态毒理效应认识水平的不断深入 ,DNA加合物的研究日益受到重视 .本文首先对DNA加合物的毒性机理与DNA加合物形成过程进行了分析 ;介绍了DNA加合物现有的诊断方法 ,包括色谱 质谱法、3 2 P 后标记法、免疫学法和荧光测定法 ;最后对DNA加合物的污染暴露指示进行了论述 ,指出DNA加合物作为有效的分子生物标记物是生态毒理学家用来预测污染物危害效应的警示信号 .

With the cognitive penetration of ecotoxicological effects into a molecular level,more attention has been paid to the research on DNA adducts.This paper analyzed toxic mechanisms and formative processes of DNA adducts,and introduced in brief their diagnostic methods,including chromatogram-mass spectrum method, 32P post-labeling method,immunology method and fluorescence method.The pollution-exposure indication of DNA adducts was expounded as an effective molecular biomarker,and thus,DNA adducts could be used by ecotoxicologists as a good caution signal in forecasting hazardous effects of pollutants.