EB病毒在鼻咽癌发生中的作用

Using PCR method to study the EB virus DNA in NPC

目的 :探讨鼻咽癌 (NPC)组织及患者外周血白细胞和血清中EB病毒DNA的分布及其与NPC的关系。方法 :采用多聚酶链反应 (PCR)检测 39例NPC组织 (NPC组 1)中EB病毒DNA ,2 4例NPC患者 (NPC组 2 )外周血白细胞及血清中EB病毒DNA ,2 0例慢性鼻咽炎组织 (对照组 1)中EB病毒DNA ,10例头颈部其他部位肿瘤患者 (对照组 2 )和 10例慢性鼻咽炎患者 (对照组 3)外周血白细胞及血清中EB病毒DNA ;同时采用免疫酶法检测NPC组 2、对照组 2和对照组 3血清中EB病毒VCA IgA抗体效价。 结果 :NPC组 1EB病毒DNA阳性率为 71.8% (2 8/ 39) ,对照组 1阳性率为 15 .0 % (3/ 2 0 ) ,其差异有统计学意义 (P <0 .0 5 ) ;NPC组 2外周血白细胞中EB病毒DNA阳性率为 4 1.6 7% (10 / 2 4 ) ,对照组 2阳性率为 10 .0 0 % (1/ 10 ) ,对照组 3外周血白细胞中EB病毒DNA为阴性 ,NPC组 2分别与对照组 2和对照组 3比较 ,差异均有统计学意义 (均P <0 .0 5 ) ;NPC组 2、对照组 2和对照组 3血清中EB病毒DNA均为阴性 ;NPC组 2血清中EB病毒VCA IgA抗体效价分别与对照组 2和对照组 3比较 ;差异均有统计学意义 (均P <0 .0 1)。结论 :NPC组织中检测出EB病毒DNA的阳性率较高 ,外周血白细胞中阳性率较低 ,血清中未能检测出EB病毒DNA 。

Objective:To study the EB virus DNA distribution in paraffin-embedded tissue,peripheral blood leukocyte and serum in nasopharyngeal carcinoma patients(NPC).Method:PCR method, in paraffin-embedded tissue of 39 patients with NPC(NPC group-1)and 20 patients with chronic nasopharyngitis(control group-1),in peripheral blood leukocyte and serum of 24 patients with NPC(NPC group-2)and 10 patients with other head and neck carcinoma(control group-2) and 10 patients with chronic nasopharyngitis (control group-3) were used to test the presence of Epstein-Barr virus DNA . At the same time, we also measured serum EB virus VCA-IgA antibodies with enzyme-immunoassay in NPC group-2, control group-2 and control group-3.Result:The results showed that 28 of 39 nasopharyngeal carcinoma patients( 71.8%)(NPC group-1) and 3 of 20 chronic nasopharyngitis(15%)(control group-1) were EB virus DNA positive in paraffin-embedded tissues. The difference between NPC group-1 and control group-1 was statistically significant. Ten of 24 NPC group-2 ( 41.67%) and 1 of 10 control group-2(10%) were EB virus DNA positive in peripheral blood leukocyte.Control group-3 EB virus DNA was negative in peripheral blood leukocyte. The difference between NPC group-2 and control group-3 was statistically significant(P< 0.05).All of the patients' EB virus DNA were negative in serum. The difference between NPC group-2 and control group-2;2 was statistically significant in VCA-IgA titer respectively(Ridit Tes P< 0.01).Conclusion:EB virus DNA positive in paraffin-embedded tissues is higher than that in peripheral blood leukocyte in nasopharyngeal carcinoma patients. In serum EB virus DNA is negative. The results suggest that EB virus DNA positive from paraffin-embedded tissues to peripheral blood leukocyte to serum is decrease gradually.There must be some relationship between VCA-IgA in serum and EB virus DNA in peripheral blood leukocyte.