复发性鼻息肉组织中mRNA差异显示分析

The recurrent nasal polyp analysed by mRNA defference to demonstrate

目的 :寻找复发性鼻息肉的相关基因。方法 :对 3例鼻息肉病患者的鼻息肉组织应用mRNA差异显示逆转录PCR(DD RTPCR)技术筛选出相关基因 ,并对差异片段进行序列分析和鉴定。结果 :在背景一致的条件下比较正常鼻甲黏膜组织与鼻息肉组织两者的扩增产物 ,在 15 0 70 0bp处共发现 11个差异片段 ;其中有 1个片段 (X 1)与第 8号染色体的长臂的一段序列完全同源 ,其上游与下游存在锌指蛋白基因。该片段在鼻息肉组织有高表达。结论 :以PCR为基础 ,用DD RTPCR技术能有效地比较鼻息肉与正常鼻甲黏膜组织或细胞间的差异表达基因 ,可筛选出鼻息肉组织相关基因。与第 8号染色体的长臂的一段序列完全同源的差异片段 。

Objective:To look for the correlated gene of nasal polyp.Method:The nasal polyp was analysed by mRNA defference to demonstrate,and the fragment of writing was analysed and appraised.Result:The amplification products of normal conchae tissue were compared with the nasal polyp tissue under the condition of background with one accord.The total 11 difference fregments were discovered during the 150-700 bp.One of the fragments(X-1) was completely homology with the section series of the eight chromosome long arm.There was a zine finger protein in the upper stream and the down stream.The fragment expressed in nasal polyp tissue.Conclusion:Base on polymerase chain reaction(pcr),using differential display R.T.PCR(DD-RTPCR) technique,the difference express gene of nasal polyp could be effectively compared with normal nasal conchae tissue or cells,the correlated gene of nasal polyp tissue could screened out.The fragment(X-1) that was completely homology with the section series of the eight chromosome long arm may be a new identified gene which cause the nasal polyp.