BTB/POZ结构域蛋白GRP对热休克中hsp900α基因表达的增强作用

Enhanced Action of a BTB/POZ Domain Protein on the Expression of hsp90α Gene in Heat Shock

目的 探讨人BTB/POZ结构域GAGA元件结合相关蛋白(GRP)在hsp90α基因表达中的作用。方法 用正义或反义GRP真核表达质粒或空载体,分别与装有hsp90α基因启动子(-1756-+37)的pREP4 episomalvector质粒和对照质粒pMCAT共转染Jurkat细胞,提取总RNA,用竞争性半定量RT-PCR测定hsp90α-氯霉素乙酰基转移酶(CAT)报告基因相对启动子活性。结果 热休克时,GRP明显促进pREP4 episomal vector质粒上hsp90α-CAT报告基因启动子活性。结论 GRP可能通过参与染色质动态调整促进pREP4 episomal vector质粒上hsp900α CAT的表达。

Objective To study the role of a BTB/POZ domain protein in the expression of hsp90α gene. Methods The eukaryotic expression plasmids of sense- and antisense-GAGA related protein (GRP) or empty vector were transfected into Jurkat cells with pREP4 episomal vector plasmids carrying the hsp90α promoter sequence from -1756 to +37 and control plasmids pMCAT. Total RNA was extracted. The relative promoter activity of hsp90α-CAT reporter gene was determined by competitive RT-PCR assay. Results GRP markly increased the relative promoter activity of hsp90α-CAT reporter gene during heat shock. Conclusion GRP may promote the expression of hsp90α gene by participating in chromatin remolding.