应用RT-PCR法快速检测马铃薯X病毒

Rapid Detection of Potato Virus X by RT-PCR

根据马铃薯X病毒的外壳蛋白基因序列 ,设计合成了 1对寡核苷酸引物 ,从感染PVX的马铃薯病叶组织中提取出病毒的RNA ,进行cDNA合成和PCR扩增 ,得到一条长度约为0 .72kb的特异性PCR扩增产物 ,与理论设计的外壳蛋白基因大小一致。在基因水平上为PVX的检测提供了一种快速、灵敏、简便的新方法 ,为PVX的防治提供了有效手段。

In lab test a pair of DNA primers were designed and synthesized based on the nucleotide sequence of the coat protein (CP) gene of potato virus X (PVX). The PVX RNA was directly extracted from virus-infected potato leaves, and utilized for cDNA synthesizing, then it was amplified by polymerase chain reaction (PCR). A specific PCR fragments about 0.72kb thus was obtained, and it was the expected length of PVX CP gene. It provides a rapid, sensitive and convenient means that can detect and identify PVX in gene level.