树月季'雪山娇霞'的离体快繁技术研究

Rapid Propagation of Tree Rose' Xueshan jiaoxia' in Vitro

对我院自育的优良树月季品种‘雪山娇霞'的离体快繁技术进行了研究.试验表明,最佳的外植体是春季的越冬芽.最佳的启动培养基是MS+6-BA0.5～1.0 mg/L+NAA0.05～0.1 mg/L,其诱导率可高达96%,继代培养基用MS+6-BA1.0 mg/L+NAA0.1 mg/L时,可保证较高的增殖率和良好的生长状况,IBA生根效果优于NAA和IAA,以1/2MS+IBA0.1～0.3mg/L为最佳.组培苗的瓶外生根可降低生产成本并缩短繁殖周期.

Rapid propagtion of tree rose' Xueshan jiaoxia'in vitro was studied. The experiment showed that the optimum explant was the axillary buds on branches after dormancy. The best medium for the shoots was MS +6-BA 0.5 -1.0 mg/L + NAA0. 05 - 0. 1 mg/L, the max shooting rate could reach 96% , the best proliferation medium was MS+6-BA1.0 mg/L + NAA0. 1 mg/L,IBA was better than NAA and IAA for rooting,and 1/2MS+IBA0. 1 -0.3 mg/L was optimum. Rooting of plantlets out of bottle could reduce cost and shorten period of propagation.