内毒素诱导感染性休克动物模型的建立及血清TNF-α测定

Lipopolysaccharide induced animal model of septic shock and detection of its serum TNF-α

目的 :建立感染性休克动物模型 ,观察病理改变。方法 :大鼠经股静脉注射内毒素 (LPS) ,观察动物的平均动脉压 (MAP)、心率变化及存活率。同时测定血清肿瘤坏死因子 (TNF α)水平评定模型。结果 :大鼠MAP于注射LPS后迅速下降 ,升至正常后逐渐缓慢下降直至死亡 ;心率短暂上升后逐渐下降 ;病理改变主要表现为间质性肺炎 ;血TNF α在LPS注射 90min升至最高 ,以后逐渐下降。结论 :应用LPS建立的感染性休克动物模型MAP、心率变化与临床感染性休克相似 ,是一个理想的模型。

Objective: To establish the animal model of septic shock and observe the pathological changes. Methods: Li popolysaccharide(LPS) of E Coli was injected to Wistar rats via femoral vein to induce septic shock, and the changes of mean arterial pressure (MAP), heart rat es and survival rate were observed. Tumor necrosis factor α (TNF-α) level was detected to evaluate the animal model. Results: The MAP decre ased obviously after injection of LPS, then recovered to normal, and decreased s lowly till death. Heart rate increased transiently, then decreased slowly. Patho logical changes presented interstitial pneumonia. Serum TNF-α reached peak at the 90th minute, then decreased gradually. Conclusion: The LPS -induced septic shock resembles the clinical septic shock. Injection of LPS can be used to establish the animal model of septic shock.