蛇毒神经生长因子抗肿瘤体外实验研究

In vitro experimental study of the antitumor potency of snake venom nerve growth factor

目的 :探讨蛇毒神经生长因子 (NGF)对人肿瘤细胞增殖的抑制作用。方法 :采用噻唑蓝 (MTT)法、台盼蓝拒染法和集落形成作为观察指标 ,以NGF 5 ,10 ,15 ,2 0 μg /ml处理 4种人肿瘤细胞 (人白血病细胞株K 5 6 2、人胰癌细胞株JF 30 5 ,人肺癌细胞株AGZY 83a和人胃癌细胞株MG 80 3) ,选用不同作用时间 ,分别观察NGF对上述细胞的影响。阿霉素 (Adr)为阳性对照药。结果 :NGF对 4种人肿瘤细胞均有细胞毒性作用 ,K 5 6 2和JF 30 5比后两者显示出更好的量效关系 (P均 <0 .0 5 )。NGF处理 2 4 ,4 8和 96h后K 5 6 2和JF 30 5细胞的生长均受到抑制 (P <0 .0 5 ,P <0 .0 1)。并有显著的剂量效应关系。处理JF 30 5细胞 2 4 ,4 8和 96h ,NGF的半抑制浓度 (IC50 ) ,时间 效应关系亦明显 ;NGF对K 5 6 2细胞的生长抑制作用以 4 8h为显著。NGF还可显著抑制JF 30 5细胞的集落形成 ,并有明显的量效关系 (P <0 .0 1) ,其IC50 为 12 .86 μg/ml。 结论 :NGF对 4种人肿瘤细胞均具有细胞毒性和生长抑制作用 ,对K 5 6 2、JF 30

Objective: To observe the inhibitory effects of nerve growth factor(NGF) on human tumor cell lines. Methods: The MTT colorimetric method, trypan blue exclusion,and colony formation was u sed. Adr used as positive control. Four kinds of human tumor cell lines, K 562,J F 305, AGZY-83a and MG-803 were treated with NGF at different final concentrat ions of 5,10,15, and 20 μg/ml. The effects of NGF on the cells mentioned above were observed at different intervals after the cell were exposed to NGF. Results:NGF had obvious cytotoxicity on these cell lines. There were obvious dose-response relationship in test of K 562 and JF 305 cells (P< 0.05). K 562 and JF 305 were exposed to NGF at different intervals of 24,48, and 96 h, the growth of both cell lines was inhibited obviously and the dose-r esponse relationships were showed distinctively. At 24, 48, and 96 h intervals a fter JF 305 cells were exposed to NGF,there was obvious time-reponse relationsh ip. The inhibitory effect of NGF on the growth of K 562 reached peak at 48h time point. NGF also possessed inhibitory effect on colony formation of JF 305 cells with satisfied dose-response relationship (P<0.01)and the IC 50 was 12.83 μg/ml. Conclusion:NGF possesses distinct cytotoxicity and growth inhibitory effect on the four kind of human tumor cells especially o n K 562 and JF 305