膀胱癌肿瘤血管生成与肿瘤细胞凋亡和增殖的关系

Relationship between tumor angiogenesis and tumor cell apoptosis and proliferation in bladder cancer

目的 :探讨膀胱癌组织中微血管密度 (MVD)与肿瘤细胞凋亡和增殖的关系。方法 :应用S P法对 6 4例膀胱癌组织中血管内皮细胞的第Ⅷ因子抗原和ki 6 7抗原进行染色 ,计数肿瘤的微血管数及ki 6 7标记指数 (LI) ;应用TUNEL法检测膀胱癌细胞凋亡状态。结果 :MVD在不同病理分期和细胞学分级上有显著差异 (P <0 .0 5 ) ,且随着病理分期和组织学分级的增高而增高 (P <0 .0 5 )。MVD与凋亡指数 (AI)呈负相关 (P <0 .0 0 1) ,与ki 6 7LI无关(P >0 .0 5 )。结论 :膀胱癌肿瘤血管生成与膀胱癌浸润、转移密切相关 ;血管生成可以抑制肿瘤细胞的凋亡 。

Objective: To discuss the relationship betwe en the tumor angiogenesis and tumor cell apoptosis and proliferation. M ethods:Immunohistochemical method was used to stain the Ⅷ factor antig en in the vascular endothelial cells and the ki-67 antigen in 64 cases of bladd er transitional cell carcinoma (TCC). The apoptotic index (AI) used in situ labe ling apoptotic DNA fragment kit and ki-67 labeling index (ki-67) and microvess el density (MVD) were measured in 64 cases of bladder cancer. Results: The MVD was significantly different with pathological stages and histol ogical grades of the tumors (P<0.05).With Spearman Rank correlation with MVD, statistical analysis revealed an inverse correlation between AI and MVD ( P<0.001)and no significant correlation was found between MVD and ki-67( P>0.05). Conclusion: Tumor angiogenesis might contribute t o tumor malignant progression by reducing tumor cell apoptosis in bladder TCC.