bcl-2,MDR1基因及编码蛋白在儿童急性白血病骨髓单个核细胞中表达的研究

Expression of bcl-2 and MDR1 genes and their proteins in bone marrow cells from patients with childhood acute leukemia

目的 :探讨bcl 2 ,MDR1基因及编码蛋白p2 6 ,p170表达与多药耐药的关系。方法 :RT PCR技术、S P免疫组化方法检测 36例白血病患儿及 10例血小板减少性紫癜患儿骨髓单个核细胞bcl 2 ,MDR1基因及蛋白表达。结果 :bcl 2基因的表达初治组、完全缓解组低于复发组 (P <0 .0 5 ) ;MDR1基因的表达复发组高于对照组 (P <0 .0 5 ) ,p2 6 ,p170阳性率初治组、完全缓解组、对照组低于复发组 (P <0 .0 5 )。p170阳性率初治组高于对照组 (P <0 .0 5 )。化疗后p170阳性率明显高于化疗前 (P <0 .0 1)。bcl 2 ,MDR1基因的表达与临床生物学特征无相关性。bcl 2与MDR1基因之间、p170和p2 6蛋白之间均无相关性。结论 :bcl 2和MDR1基因、蛋白通过不同的机制与白血病的耐药有关。

Objective: To explore expression of bcl-2 and (multidrug resistance 1) MDR1 genes and their proteins p26,p170 in childhood with acute l eukemia. Methods: The expression levels of bcl-2, MDR1 were e valuated by using RT-PCR and S-P immunohistochemical method. Results: The expression of bcl-2 in incipient group and complete remission gro up were much lower than that in relapsed group(P<0.05). The expression of MDR 1 relapsed group was much higher than that in control group(P<0.05). T here was much higher expression of MDR1 after induction chemotherapy(P< 0.0 1). The expression of p26 and p170 in relapsed group were much higher than t hose in incipient group, complete remission group,and control group(P<0.05 ). The expression of p170 in incipient group was much higher than that in contr ol group(P<0.05). After induction chemotherapy, p170 expression was much higher. There was no significant relationship between the levels of bcl-2, MDR 1 genes and clinical features. There was no relationship between bcl-2 and MDR1 genes, p26 and p170. Conclusion: bcl-2 and MDR1 genes and th eir proteins are related to drug resistance according to different mechanisms.