葛根芩连汤对抗地塞米松诱导大鼠胰岛素抵抗作用

Effects of Gegenqinlian Decoction on Antagonizing Insulin Resistance in Dexamethasone-induced Rats

目的 研究葛根芩连汤时地塞米松(dexamethasone,DEX)诱导大鼠胰岛素抵抗模型动物糖耐量的效应,同时观察丙二醛(MDA)及超氧化物歧化酶(SOD)的影响,初步探讨其降低动物血糖的作用机制。方法 以DEX诱导胰岛素抵抗(insulinresistance,IR),糖耐量减退(IGT)大鼠模型,测其空腹血糖(FBG)和口服葡萄糖耐量试验(OGTT)后2 h血糖(2 hBG)以及给药后2 h空腹血清血糖(FSG)、SOD活性和MDA含量。结果 该方降低病鼠FBG和OGTT后2 hBG(P<0.01),改善DEX致IR模型大鼠的IGT;降低FSG(P<0.05),但对SOD和MDA无显著影响(P>0.05)。DEX抑制SOD的活性(P<0.01),并使MDA的含量升高(P<0.01)。结论 葛根芩连汤具有磺脲类药物的降糖作用。地塞米松致氧自由基清除受阻,导致机体脂质过氧化损伤,但方剂未显示对抗作用。

Objective To investigate the effects of Gegenqinlian( GGQL) decoction on improving insulin resistance(IR) and oxidation-antioxidation in dexamethasone( DEX) -induced insulin resistant rats. Methods To study GGQL decoction 9.0 g/kg, intragaslroesopha-geally administered, qd×8d,on DEX(1.0 mg/kg intramuscularly injected, qod ×4d)-induced impaired glucose tolerance( IGT) IR rats that fasting blood glucose (FBG) ,2-hours blood glucose (2hBG) after oral glucose tolerance test(OGTT) ,fasting serum glucose (FSG) ,the content of malondialdehyde( MDA) and activity of superoxide dismutase( SOD) were observed. Results DEX induced IR, inhibited activity of SOD and increased content of MDA ( P <0. 01) ;GGQL decoction lowered FBG (P<0.05) and 2hBG (P<0.01) after OGTT, improved IGT, lowered FSG (P < 0. 01 ) but didnt influence the content of MDA and activity of SOD ( P > 0. 05 ) in DEX-induced IR-rats. Conclusions The results suggest that GGQL decoction and gliclazide can improve DEX-induced insulin resistance, both mechanism can be the same, but the preparation does not show antioxidation.