血吸虫感染新模型小鼠血清IgG与抗原的免疫印迹分析

WESTERN BLOT ANALYSIS OF SCHISTOSOMA JAPONICUM ANTIGENS RECOGNIZED BY SERUM IgG FROM A NEW MODEL OF MICE

目的 在建立一种日本血吸虫感染小鼠伴随免疫新模型的基础上,比较新模型小鼠与血吸虫感染常规模型小鼠血清IgG识别日本血吸虫抗原的差异。方法 每只BALB/c小鼠经腹部皮肤人工感染(40±1)条日本血吸虫尾蚴20d后,按300mg/(kg·d)腹腔注射酚酶抑制剂-丙烯基硫脲,同时设立未用药感染对照组,至感染后第42天剖杀2组小鼠,从小鼠眼球取血制备血清,经western-blot观察血清IgG对日本血吸虫SWAP及SEA的识别差异。结果 western-blot显示,2种模型小鼠血清IgG对SWAP的识别存在差异,明显的区别在于常规模型组可见1条90kDa大小的清晰主带,而新模型组则表现为数条弱带。SEA与常规模型组小鼠血清。IgG呈强阳性反应,而与新模型组小鼠血清IgG反应微弱。结论 2种模型小鼠体内血吸虫诱导产生的抗体存在差异,这些差异可能是导致新模型小鼠抗再感染力增强的体液免疫因素之一。

Objective To compare the differentiation of Schistosoma japonicum antigens recong-nized by serum IgG between a new model of infected mice and the normal model of infected mice. Methods From the 20th day after every BALB/c mouse was infected artificially with (40±1) cer-cariea, 10 mice to be as a new model group were continually injected with allyl thiourea in a dosage of 300 mg/kg every day by intraperitoneal injection, while another 10 mice to be as a normal model group were not done so, until the 42th day, these mice all were sacrificed, and their serum were obtained by the traditional method. By means of SDS-PAGE and Western-blot, SWAP and SEA were separated and recognized by serum IgG from two mouse models separately. Results The obvious differentiation of SWAP bands were found between the two groups. The most prominent differentiation was a band of 90 kDa molecule, which was found in the normal model group but was substituted by several weak bands in the new model group. SEA were recognized strongly by the normal model group but very weakly by the new model group. Conclusion Different IgG antibodies are induced by the living worms parasitized in the mice of the new model, which may be one of reasons for a higher resistance against reinfection in the new mouse model demonstrating concomitant immunity.