摘要
目的 探讨大量人工感染钉螺的尾蚴收集及逸蚴得量。方法 在实验室条件下,用日本血吸虫毛蚴人工感染钉螺,60d后把全部感染钉螺分7份,每天逸1份,每10天对各份钉螺进行1轮逸蚴,用低速离心方法收集尾蚴,以沉淀尾蚴的重量作为尾蚴得量。结果 1900g贵池江滩钉螺,感染后经120d常规实验室饲养,感染率为36.00％,存活率为51.58％,经6轮40个逸蚴日的逸蚴,共收集尾蚴10.5g,经推算逸蚴期每1000只阳性钉螺1次逸蚴可获得尾蚴0.2573g。
Objective To explore the Schistosoma japonicum cercariae collected method and the quantity of cercariae obtained from a great quantity artificial infected snails. Methods In laboratory condition, Oncomelania snails were infected with schistosome miracidia. Sixty days post-infection all snails were divided into 7 shares. Cercariae shed from 1 share snails every day and the number of all shedding days were 40. Cercariae shed from snails were collected with low-velocity centrifuge and the cercariae sediment were weighted. Results One thousand and nine hundred g snails bred for 120 days post-infection, the infection and survival rates were 36. 00% and 51. 58%. Cercariae col-lected were 10. 5 g from 40 days collection. Cercariae quantity of shedding from 1 000 positive snails per day was 0. 257 4 g.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
2003年第6期448-449,共2页
Chinese Journal of Schistosomiasis Control
基金
江苏省科技厅社会发展科技计划项目[No.BS2001061]
江苏省地方病协会血寄地科研项目[No.X2113]
关键词
日本血吸虫
尾蚴
人工感染钉螺
逸蚴
得量
Schistosoma japonicum cercariae
Artificial infected snails
Shedding cercariae
Cercariae quantity
