α-氰基丙酸酯胶体内固定放射性碘化油的实验研究

Experimental study of ZT glue fixing radioiodinated oil in vivo

目的 探讨α-氰基丙酸酯(ZT)胶体内固定放射性碘化油的可能性。方法 系列体积比例的ZT胶与碘化油体外混合加生理盐水,观察30min后凝固与否。V(ZT胶):V(^(131)I-碘化油)=1:4体外混合,按503.2 kBq(0.1ml)/只皮下注射5只SD大鼠,连续γ显像观察注射点的γ计数;38 d处死、解剖动物,观察注射点碘化油的状态,分别取心、肺、肝、脾、肾、睾丸、右股骨、注射点组织测量γ计数。另5只SD大鼠注射等量^(131)I-碘化油做对照。结果 1:10以上体积比的ZT胶和碘化油均可在30 min内完全凝固。ZT胶组注射点^(131)I-碘化油呈淡黄色豆渣样,由薄膜包裹;无ZT胶组局部呈脂肪增生;均无明显炎症反应。ZT胶组碘化油生物半排期是无ZT胶组的4倍,生物半排期单因素方差分析F=81.999,P<0.001;有效半排期比较F=14.158,P=0.006。38 d后局部放射性残留ZT胶组明显较多,两组单因素方差分析F=5.608,P=0.045。ZT胶组正常器官内放射性38 d滞留量与无ZT胶组相比,肝脏差异有显著性(F=6.928,P=0.03),余器官差异均无显著性。结论 ZT胶体内固定放射性碘化油是可能的。

Objective To study the possibility of α-cyano-acrylic acid (ZT) glue fixing radioiodinated oil in vivo. Methods ZT glue and iodinated oil mixture of a series of volume ratios were mixed with saline, and the coagulation process within 30 min was observed. A mixture with 1:4 volume ratio of ZT glue to 131 I-iodinated oil was injected subcutaneously into 5 rats, 503.2 kBq (0.1 ml)/rat. Continuous SPECT was performed to observe γ counts at injection sites. After 38 d, animals were put to death and dissected. Local iodinated oil condition, γ counts of the heart, lung, liver, spleen, kidney, testis, right femur and injection site were recorded. Another 5 rats were injected131 I-iodinated oil without ZT glue as control group. Results ZT glue and iodinated oil mixed as 1:10 volume ratio or higher could coagulate completely within 30 min. At 38 days after injection,131 I-iodinated oil at the injection sites of the experimental group looked yellowish with a texture similar to bean dreg, and coated by a film-like substance; the injected131 I-iodinated oil of the control group was replaced by fat tissue. Both of them had no inflammation reaction. The biological half drain time of the iodinated oil of the experimental group was 4 times longer than that in control group with the F value of 81.999, P < 0.001. Effective half decrease time values of 131 I-iodinated oil in two groups were different from each other with the F value of 14.158, P = 0.006.After 38 d, the nuclide remainder at the local site in the experimental group was more than that in control group with the F value of 5.608, P = 0.045. The nuclide remainder in the specimens of other organs in the experimental group was similar to that in control group except in the liver specimen which was a little higher. Conclusion It is possible that using ZT glue to fix radioiodinated oil in vivo.