银杏叶提取物(GBE)对大鼠局灶性脑缺血后细胞间黏附分子-1及其mRNA表达的影响

Effect of Ginkgo biloba extract on intercellular adhesion molecule-1 and mRNA expression during cerebral ischemia/ reperfusion in rats

目的 探讨银杏叶提取物对鼠脑缺血后细胞间黏附分子 1(ICAM 1)及其mRNA表达的影响。方法 建立大鼠大脑中动脉闭塞 /再灌注模型 ,应用RT PCR及免疫组织化学的方法 ,观察各实验组鼠脑缺血后细胞间黏附分子 1及其mRNA的表达。结果 ICAM 1及其mRNA在假手术组鼠脑组织呈低表达 ;缺血 90min再灌 2 4h脑组织ICAM 1及其mRNA表达较前显著增高 (P 0 .0 1) ;银杏叶提取物治疗组于相同时限ICAM 1及其mRNA的表达与缺血再灌注组相比较显著下调 (P 0 .0 1)。结论 银杏叶提取物能显著下调ICAM 1及其mRNA的表达 ,减轻脑缺血后炎性病理损害 ,具有明显的缺血脑保护作用。

OBJECTIVE To investigate the effect of Ginkgo biloba Extract(GBE) on intercellular adhesion molecule 1(ICAM 1) and mRNA expression following cerebral ischemia/reperfusion in rats. METHOD The middle cerebral artery (MCA) focal cerebral ischemia/reperfusion rat models were used. The ICAM 1 and mRNA expression were measured in groups by RT PCR and immunohistochemistry techniques,respectively.RESULTS Lower of ICAM 1 and ICAM 1 mRNA expression was observed in pseudo surgery group,and the expression of ICAM 1 and ICAM 1 mRNA distinctly up regulated in the ischemia/reperfusion group. A significant reduction in the expression of ICAM 1 and ICAM 1 mRNA was found in the GBE treated group compared with the ischemia/ reperfusion group following focal cerebral ischemia and reperfusion in rats.CONCLUSION GBE may alleviate inflammation in cerebral ischemic region and exert a potent effect on cerebral ischemia/ reperfusion by down regulating the expression of ICAM 1 and ICAM 1 mRNA.