肝硬化患者血浆转化因子TGF-β1测定的意义

Significance of transforming growth factor-β1 in patients with liver cirrh osis following chronic hepatitis B

目的:探讨肝硬化患者血浆转化因子β1(TGF-β1)的变化及其意义。方法:经病史、临床生化、B超检查确诊的72例乙肝后肝硬化患者,依据child-pugh分级分为3级,其中A级23例,B级29例,C级20例。健康志愿献血者6例做为正常对照组。ELISA法测定血浆TGF-β1的变化。并同时检测血透明质酸酶(HA)、Ⅳ型胶原(CⅣ)、Ⅲ型前胶原(PCⅢ)、血浆白蛋白、凝血酶原时间及其活动度、胆红素改变。结果:随着肝硬化的进展,血浆TGF-β1、血HA、CⅣ、PCⅢ逐渐增加。childB、childC级患者血浆TGF-β1与血HA、PCⅢ均明显增加。血浆TGF-β1与血HA呈正相关(childB组r=0.51,P<0.01;childC组r=0.67,P<0.01),与血PCⅢ呈正相关(childB组r=0.63,P<0.05;childC组r=0.74,P<0.01)。结论:血浆TGF-β1可反映肝纤维化程度,联合HA、PCⅢ检测可用作肝硬化诊断、追踪观察的有意义指标。

Objective:To study the relationship between the plasma l evels of TGF-a1and the serum hyaluronic acid(HA)and typeⅢprocollagen(PCⅢ )in patients with different stages of liver cirrhosis following chronic hepati tis B.Methods:72cases with liver cirrhosis of different stages were classif ied according to child-pugh`s categories A,B,C,in which there were23cases in child A,29cases in child B and20cases in child C.The plasma levels of TGF-a1and the serum levels of HA,(collagen typeⅣ,CⅣ)were determined by ELISA.The serum PCⅢconcentration was determined by radioimmunoassay.Results :With the progression of hepatic fibrosis,the plasma levels of TGF-a1and t he serum levels of HA,CⅣ,PCⅢwere all in creased and the highest levels were in child C.There was positive correlation between the plasma levels of TGF-a 1and the serum HA,in child B(r=0.51,P<0.01)and in child C(r=0 .67,P<0,01).The plasma levels of TGF-a1revealed a significant co r-relation with the serum PCⅢin child B(r=0.63,P<0.01)and in chil d C(r=0.74,P<0.01).Conclusion:The plasma levels of TGF-a1was related to the progression of liver cirrhosis.TGF-a1plays a role in liver ci rrhosis.