抽动障碍患儿免疫功能检测与其临床意义

A Study on Immune Functional State and Clinical Implications in Children with Tic Disorder

目的 观察抽动障碍患儿外周血 T淋巴细胞亚群、自然杀伤细胞和体液免疫 ( Ig A,Ig G,Ig M)功能。方法 应用直接免疫荧光染色法 ,采用流式细胞术分别测定 3 0例抽动障碍患儿和 2 0名健康对照组儿童外周血 T淋巴细胞亚群、自然杀伤细胞。并用免疫透射比浊法测定患儿的体液免疫 ( Ig A、Ig G、Ig M)功能。比较两组的检测结果。结果 抽动障碍患儿外周血 CD4+细胞百分比、CD4+ /CD8+细胞比值和自然杀伤 ( NK)阳性细胞百分比分别为 ( 2 6.89± 9.0 8) %、( 0 .88± 0 .3 5 ) %、( 9.61± 6.45 ) % ,较对照组 ( 3 8.3 1± 6.95 ) %、( 1 .5 8± 0 .2 9) %、( 1 4.83±4.1 6) %明显降低 ,CD8+ 细胞百分比 [( 3 2 .5 8± 9.0 4) % ]较对照组 [( 2 4.82± 5 .5 4) % ]明显升高 ,而体液免疫Ig A、Ig G、Ig M含量与对照组比较则差异无显著性。结论 抽动障碍患儿存在细胞免疫功能紊乱 ,表现为 T淋巴细胞亚群平衡失调和自然杀伤细胞阳性率低。提示细胞免疫功能紊乱可能与某些儿童易患抽动障碍有关。

Objective To explore the immune state of T lymphocyte subgroups, natural killer cell(NKC) and immunoglobulin(Ig) in children with tic disorder(TD). Methods The proportion of lymphocytic subsets,NKC,IgA,IgG and IgM in peripheral blood were measured in 30 cases of TD and 20 healthy children. Results The levels of CD4+,CD4+/CD8+ and NKC in children with TD were (26.89±9.08)%,(0.88±0.35)%, (9.61±6.45)%, which showed significantly depressed, as compared to that in healthy controls[(38.31±6.95)%,(1.58±0.29)%,(14.83±4.16)%]. The levels of CD8+[(32.58±9.04)%] in TD were significantly increased, as compared to that in healthy controls[(24.82±5.54)%]. But the levels of IgA, IgG, IgM in patients with TD were not significantly different from the healthy children. Conclusions The present study demonstrates that the cell immune functional state of T lymphocytes and NKC in children with TD were at low level underlying the disease.