大鼠膀胱经背段的神经解剖学特征——CB-HRP逆行示踪研究

Neuroanatomical Specificities of the Dorsal Trunk Segment of the Bladder Meridian--A Study of Retrograde Tracing of CB-HRP

目的 通过对大鼠膀胱经躯干段经过的背阔肌及背最长肌的相应脊髓和背根神经节内标记神经元的研究 ,了解经脉线及非经脉线神经支配的区别和经络的神经解剖学特征 ,从而为经络实质的研究提供形态学基础。方法 用霍乱毒素 B亚单位结合辣根过氧化物酶 ( CB-HRP)对膀胱经经脉线及非经脉线上背阔肌和背最长肌进行逆行示踪研究。结果 经脉线上脊髓腹角运动神经元和背根节感觉神经元标记细胞数明显多于非经脉线 ,并且经脉线上脊髓腹角运动神经元有较为广阔的树突野。结论 在经脉线所通过的背阔肌及背最长肌中 ,感觉及运动神经终末密集 ,经络的功能可能与丰富的传入传出神经支配有关。

Objective The purpose of this research was to provide neuroanatomical basis for Meridian by studying the differences of the labeled neurons supplying Latissimus Dorsi Muscle and Erector Spinae Muscle located at the dorsal trunk segment of Bladder Meridian and at non-Meridian in rats. Methods Cholera toxin B subunit conjugated horseradish peroxidase (CB-HRP) was used to label the neurons innervating Latissimus Dorsi Muscle and Erector Spinae Muscle at the dorsal trunk segment of Bladder Meridian and at non-Meridian. Results (1)There are statistically significant differences between Bladder Meridian and non-Meridians in the numbers of CB-HRP labeled neurons in spinal ventral horns and in dorsal ganglia innervated the two muscles. The labeled neurons at Bladder Meridian are much more than those at non-Meridians. (2) The motoneurons innervating the two muscles at dorsal trunk segment of Bladder Meridian showed much wider dendritic field and more dendritic branches than these at non-Meridians and the longitudinal dendritic bundles of motoneurons were more often observed at Bladder Meridian than at non-Meridian. Conclusions These findings indicate that the neuroanatomicalbasisofMeridianisprobablyrelatedtoitsricher innervation and widespread connection of central neurons.