聚乳酸-甲壳素接骨板的体外细胞毒性研究

Cytotoxicity Evaluation of Polylactic Acid-Chitin Plates

目的:利用体外细胞培养技术,对聚乳酸.甲壳素接骨板的细胞毒性进行研究,为临床应用作前期准备。方法:将材料浸提液与小鼠成纤维细胞接触2、4、7 d后,用分光光度仪在588 nm波长处测定吸光度,用细胞增殖度试验法评价材料的细胞毒性;采用细胞形态学试验法,在倒置显微镜下观察材料与小鼠成纤维细胞共培养时,细胞形态学发生的变化,评价材料的细胞毒性。结果:该材料细胞毒性为0级,无细胞毒性。结论:聚乳酸-甲壳素接骨板具有良好的细胞相容性。

Objective:To evaluate the cytotoxicity of Polylactic Acid-Chitin Plates and provide a sound scientific basis for clinical use. Method; The in vitro cytotoxicity of Polylactic Acid-Chitin Plates was examined by cell relative growth rate (RGR) assay and cell morphology test. Results; there was no cytotoxicity. Conclusion; The material is compatible with the tested cells.