摘要
采用修饰猪血红蛋白为氧载体构建心脏保存液,并用于体外低温保存家兔心脏组织.通过电镜观察心肌细胞超微结构的变化,比较了Krebs Henseleit保存液及其经3,5 二溴水杨酸双延胡索酸酯修饰猪血红蛋白(DBBF pHb)及3,5 二溴水杨酸双延胡索酸酯(DBBF)和聚乙二醇双重修饰猪血红蛋白(mPEG DBBF pHb)改良的保存液.结果显示,mPEG DBBF pHb改良保存液的保存效果最佳,保存24h后心肌细胞核形态规则,肌节清晰,肌丝排列整齐,线粒体结构略有肿胀,但与对照组最为接近.
Modified porcine hemoglobin was used to develop new cardiac storage solutions to preserve rabbit myocardial tissue. Ultrastructure of cardiac myocytes was observed with electron microscope. The efficacy of KH solution, DBBFpHb solution and mPEGDBBFpHb solution for cardiac preservation was evaluated. The results showed that mPEGDBBFpHb solution was the best, and the cardiac myocyte has a regular nuclear shape, clear sarcomere, good organized myofibrils, and only slightly swollen mitochondria after 24 hours of storage.
出处
《科技通报》
北大核心
2003年第3期183-186,191,共5页
Bulletin of Science and Technology
基金
国家自然科学基金资助项目(No.39880006)
邵逸夫医院医院科学基金项目(No.1200039)
关键词
医学实验动物学
心脏疾病
心脏保存液
修饰猪血红蛋白
化学修饰
电镜
超微结构
medical experimental zoology
heart diseases
cardiac storage solution
porcine hemoglobin
chemical modification
electron microscopy
ultrastructure
