耳针、艾灸对D-半乳糖致衰老大鼠血清褪黑素含量及其昼夜节律性的影响

Effects of auricular acupuncture and moxibustion on serum melatonin content and melatonin circadian rhythmicity in the D-galactose-induced aging rat

目的 :探讨耳针、艾灸延缓衰老的可能作用机理。方法 :通过皮下注射D 半乳糖建立衰老模型大鼠 ,在造模过程中 ,各治疗组分别给予耳针、艾灸、耳针 +艾灸治疗 ,治疗 6周后 ,测定各组血清褪黑素 (MT)昼夜含量、血清MT昼夜节律性振幅变化、松果体细胞数。结果 :(1)与模型组相比 ,各治疗组夜间血清MT含量增加、MT昼夜节律性振幅变大、松果体细胞数增多 ,其中以耳针 +艾灸组最为显著。 (2 )各组日间血清MT含量比较 ,差异无显著性意义 (P >0 0 5 ) ,各组血清MT含量呈现昼低夜高的特点。结论 :耳针、艾灸通过对MT及松果体的调节而起到延缓衰老的作用。

Objective To probe the mechanism of auricular acupuncture and moxibustion in delaying aging. Methods Aging rat model was established by hypodermic injection of D-galactose. In the course of establishment of the model,three treatment groups were treated respectively by auricular acupuncure,moxibustion,auricular acupuncture plus moxibustion for 6 weeks. Serum melatonin (MT) contents in day and night,changes of the serum MT and cell numbers in the pineal body were determined. Results (1) The serum MT content in the night,amplitude of circadian rhythmicity of serum MT and cell numbers of the pineal body in all the treatment groups increased as compared with the model group,with the most significant in the auricular acupuncture plus moxibustion group. (2) There no significant difference among the groups in the serum MT content in day ( P >0.05);the serum MT contents in all the groups showed the characteristics,that is,higher in the night and lower in day. Conclusion Auricular acupuncture,and moxibustion exert the action delaying aging possibly through the regulation on MT and the pineal body.